Cell-based assays, while originally used primarily for secondary screening, are now being used increasingly for primary screens. This has been tremendously valuable in screening all types of compounds and particularly those compounds for druggable cell membrane proteins, such as GPCRs, ion channels, and cell receptor kinase proteins. The success of the screening campaign is only as good as the reagents that are used in the assays.
Selexis SURE Cell Line Development™ technology can be used to generate cell lines for a whole range of cell-based assays, including enzyme and cell receptor activity assays. Cell lines that have been created with Selexis SGE® (Selexis Genetic Elements) express recombinant proteins at significantly higher levels than those that have been generated using traditional methods and exhibit higher stability. As a result, it is now possible to generate stable cell lines that express difficult-to-express biomarkers, such as GPCRs, ion channels, and cell receptor kinases, at levels that are assayable.
Cell-based assays that are built around Selexis SURE Cell Line Development technology have the advantages of:
- High expression, increasing the chances of generating cell lines with robust signals
- Expression stability over >90 generations, making it unnecessary to keep thawing fresh batches of cells
- A lower likelihood of failed screening runs due to loss of target expression in the assay
- Not requiring selective media to maintain high-level expression